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Patch pipettes (2–5 MΩ) pulled from borosilicate glass (World Precision Instruments, PG10150-4) were filled with a Cs-based low-Cl – internal solution containing (in mM) 135 CsMeSO 3 , 10 HEPES, 1 EGTA, 3.3 QX-314, 4 Mg-ATP, 0.3 Na-GTP, 8 Na 2 -phosphocreatine, 290 mOsm/kg, adjusted to pH 7.3 with CsOH
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PENS & Vials:10mg pen
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